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Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)
Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Green)
This kit can be used to measure reactive oxygen species (ROS) in fresh tissue and alive cell samples.
Catalog No:
E-BC-K138-F
Regular price
$286.00 USD
Regular price
$220.00 USD
Sale price
$286.00 USD
Unit price
/
per
2 weeks
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Product Details
Known Citations
295
Sample Type
Fresh tissue, Cultured cell
Sample Size
200-300 uL
Incubation(s)
1 hour(s) assay time
Research Areas
Oxidative Stress, Ferroptosis, Metabolic Diseases, Energy Metabolism
Background
DCFH-DA (2,7-dichlorofuorescin diacetate) is a fluorescent probe without fluorescence that can freely cross the membrane. After entering the cell, it can be hydrolyzed by intracellular esterase to form DCFH (dichlorofluorescin). In the presence of reactive oxygen species (ROS), DCFH is oxidized to DCF (dichlorofluorescein) which is a strong green fluorescent substance that cannot penetrate the cell membrane. DCF has a maximum wave peak near the excitation wavelength of 502 nm and the emission wavelength of 525 nm, and the intensity is proportional to the level of intracellular reactive oxygen species.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at -20C for 12 months away from light. Microplate can be stored at ambient temperature.
Analyte
Reactive Oxygen Species
Regulatory Status
For Research Use Only
