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Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Red)
Reactive Oxygen Species (ROS) Fluorometric Assay Kit (Red)
This kit can be used to measure reactive oxygen species (ROS) in alive cell samples.
Catalog No:
E-BC-F005
Regular price
$260.00 USD
Regular price
$200.00 USD
Sale price
$260.00 USD
Unit price
/
per
2 weeks
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Product Details
Known Citations
33
Sample Type
Cell
Incubation(s)
1.5 hour(s) assay time
Research Areas
Oxidative Stress, Ferroptosis, Energy Metabolism
Background
Reactive oxygen species (ROS) refers to the general term of substances that are composed of oxygen in the body or in the natural environment and contain oxygen and are active in nature: there is mainly a kind of excited oxygen molecule, namely singlet oxygen molecule or singlet oxygen molecule. Three oxygen-containing free radicals, namely superoxide anion radicals, hydroxyl radicals, and hydroperoxyradicals, two peroxides, namely hydrogen peroxide and lipid peroxide, and a nitrogen-containing oxide. Dihydroethidium can cross the cell membrane and enter the cell to bind to DNA or RNA, forming hydroethidium to produce red fluorescence. Dihydroethidium is mainly oxidized by superoxide anion type reactive oxygen species in cells.
Dihydroethidium itself is blue fluorescence with a maximum excitation wavelength of 370 nm and a maximum emission wavelength of 420 nm. After dehydrogenation, dihydroethidium binds to RNA or DNA to produce red fluorescence with an excitation wavelength of 300 nm or 518 nm and an emission wavelength of 610 nm.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at -20C for 12 months away from light.
Analyte
Reactive Oxygen Species
Regulatory Status
For Research Use Only
