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Recombinant Mouse HGF (C-6His)

Recombinant Mouse HGF (C-6His)

Recombinant Mouse Hepatocyte Growth Factor is produced by our Mammalian expression system and the target gene encoding Gln33­Leu728 is expressed with a 6His tag at the C-terminus.

Catalog No: EPT121
Regular price $259.00 USD
Regular price $199.00 USD Sale price $259.00 USD
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Product Details

Accession Q08048
Molecular Weight 53.5&26.9 KDa
Apparent Molecular Weight 32-38&51-59 KDa, reducing conditions
Purity Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Expression Host Mammalian
Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. 
Shipping Condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed in the Storage field.
Storage Condition and Shelf Life Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20C for 3 months.
Background HGF, is a pleiotropic protein in the Plasminogen subfamily of S1 peptidases. Mouse HGF is secreted as an inactive 728 amino acid (aa) single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide-linked HGF with a 60 kDa alpha  and 30 kDa beta  chain. HGF binds heparan-sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGF R/c-MET. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis.  It can also alter epithelium morphology by the induction of nectin‑1 alpha  ectodomain shedding, an adhesion protein component of adherens junctions .
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Regulatory Status For Research Use Only
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