• IP antibody use：5ug Flag Mouse IgG1 per ml Lysate, WB 1:5000 1. untransfected 293 cell lysate 2. transfected 293 cell lysate with Flag-tag fusion protein 3. IP (transfected 293+ normal Mouse IgG+Protein G agarose) 4. IP (transfected 293+anti- Flag mAb+ P
  • 1ug Flag fusion protein+ Primary antibody dilution at 1:10,000.
  • IF analysis of 293 cells transfected with a Flag-tag protein, using ELKbio anti-FlagTag Mouse mAb at a 1:2000 dilution (blue DAPI, red anti-Flag)
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Flag-Tag Mouse mAb

Flag-Tag Mouse mAb

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Catalog No: EM1000
$148.00 USD
$148.00 USD
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Packaging
1.5 weeks
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Product Details

Application(s) WB, IP, IF
Source Murine
Isotype IgG1
Immunogen Synthetic Peptide
Concentration 1 mg/mL
Dilution WB 1:5,000 IP 1:200 IF 1:2,000
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20C. Avoid repeated freeze-thaw cycles.
Analyte Flag-Tag
Regulatory Status For Research Use Only
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