• Human Irisin ELISA, Time 3.5 hours Sensitivity 0.11 ng/mL
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Human Irisin ELISA Kit

Human Irisin ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human Irisin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Irisin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human Irisin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Irisin in the samples is then determined by comparing the OD of the samples to the standard curve.

Catalog No: ELK10769
$458.00 USD
$458.00 USD
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2 weeks lead time
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Product Details

Species Reactivity Human
Sensitivity 0.11 ng/mL
Detection Range 0.32-20 ng/mL
Sample Type serum, plasma, tissue homogenates and other biological fluids
Incubation(s) 3.5h
Research Areas CD & Adhesion molecule
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life If the kit is not opened, store the whole kit: 4C(short time storage, valid for 6 months); -20C(long-term storage, valid for 1 year). Avoid repeated freeze-thaw cycles If the kit is opened, Store the whole kit at 4C. If the kit is not used up in 1 week. Store the Pre-Coated Microplate, Standard ,Biotinylated-Conjugate and Streptavidin-HRP at -20C, the rest reagents at 4C, please used up within 6 months.
Analyte Irisin
Regulatory Status For Research Use Only
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