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Pg (Progesterone) ELISA Kit

Pg (Progesterone) ELISA Kit

This Progesterone ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Pg. During the reaction, Universal Pg in the sample or standard competes with a fixed amount of Universal Pg on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Pg. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Pg in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Catalog No: E-EL-0154
Regular price $495.00 USD
Regular price Sale price $495.00 USD
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1.5 weeks lead-time
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Product Details

Known Citations 56
Species Reactivity Multi-Species
Sensitivity 0.15 ng/mL
Detection Range 0.31-20 ng/mL
Sample Type Serum, plasma, urine, saliva
Incubation(s) 2.5 hour(s)
Research Areas Signal Transduction
Background This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Universal Pg. During the reaction, Universal Pg in the sample or standard competes with a fixed amount of Universal Pg on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Universal Pg. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Universal Pg in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life Unopened kits should be stored at 2 to 8C until expiration. If opened, follow the instructions in the IFU. Generally speaking, if opened store the microplate, standard, detection antibody and HRP conjugate at -20C.
Analyte Progesterone
Regulatory Status For Research Use Only
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