The Apolipoprotein M (ApoM) ELISA is an enzyme immunoassay for the quantitative determination of Apolipoprotein M (ApoM) in human serum and plasma.
The microtiter plate is coated with the antibody specifically binding the Apolipoprotein M. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue color. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of Apo M in the specimen. The concentration of Apo M in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.
Catalog No:BA3007
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Product Details
Species ReactivityHuman
Sensitivity0.07 ng/mL
Detection Range0.313 - 10 ng/mL
Sample TypeSerum, Plasma
Sample Size5 uL
Incubation(s)2.5 hour(s)
Research AreasCardiovascular, Metabolism
BackgroundApolipoprotein M is a secreted 25kDa member of the lipocalin protein family. Apo M is predominantly expressed in the mature liver and kidney and plays a role in lipid metabolism. It is found associated with high density lipoproteins (HDL) in human plasma and to a lesser extent with low density lipoproteins (LDL), triglyceride-rich lipoproteins (TGLRP) and chylomicrons and is involved in lipid transport. It plays an important role in the antiatherogenic function of HDL by influencing the accumulation of cholesterol in these particles and reverse cholesterol transport. ApoM was found to increase the capacity of HDL to induce cholesterol efflux from macrophage foam cells and to inhibit LDL oxidation. ApoM also might be a useful biomarker for predicting the progression of diabetic nephropathy, and the ApoM/S1P-S1P1 axis might serve as a novel therapeutic target for preventing the development/progression of diabetic nephropathy.
