• Beta-2 Microglobulin ELISA
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Beta-2 Microglobulin ELISA

Beta-2 Microglobulin ELISA

The Beta 2-microglobulin ELISA is an enzyme immunoassay for the quantitative determination of Beta 2-microglobulin in human serum, plasma, and urine.

The microtiter plate is coated with the antibody specifically binding the Beta 2-microglobulin. The human serum, plasma or urine is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with HRP-labelled detection antibody. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue color. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of the Beta 2-microglobulin the specimen. The concentration of Beta 2-miroglobulin in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA1010
$560.00 USD
$560.00 USD $560.00 USD
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2 weeks

Product Details

Species Reactivity Human
Sensitivity 0.4 ng/mL
Detection Range 1.25 - 40 ng/mL
Sample Type Serum, Plasma, Urine
Sample Size 5 uL
Incubation(s) 3 hour(s)
Research Areas Nephrology
Background Urinary excretion of Beta 2-microglobulin (beta 2M) is a widely used test of renal proximal tubular function. Proximal tubular dysfunction leads to an increased urinary concentration.2 The serum level of beta 2-microglobulin is determined by the glomerular filtration rate and the rate of synthesis. Increased production, with raised serum levels, is sometimes observed in malignancy--mainly at an advanced state. A strong correlation between lupus nephritis (LN), disease activity, and serum beta 2-microglobulin (b2MG) was observed. Serum b2MG level can be used as a valuable predictor for LN, clinical disease activity, and damage score.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Beta-2-Microglobulin
Regulatory Status For Research Use Only
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