1
/
of
1
Bovine SCD (Acyl-CoA Desaturase) ELISA Kit
Bovine SCD (Acyl-CoA Desaturase) ELISA Kit
The Bovine (SCD) Acyl-CoA Desaturase ELISA Kit measures Acyl-CoA desaturase in Bovine samples. The plate has been pre-coated with Bovine SCD antibody. SCD present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Bovine SCD Antibody is added and binds to SCD in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SCD antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Bovine SCD. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E2185Bo
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
Couldn't load pickup availability
Product Details
Species Reactivity
Bovine
Sensitivity
9.81 ng/L
Detection Range
20-3800 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Involved in the biosynthesis of highly unsaturated fatty acids (HUFA) from the essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3) precursors, acting as a fatty acyl-coenzyme A (CoA) desaturase that introduces a cis double bond at carbon 6 of the fatty acyl chain. Catalyzes the first and rate limiting step in this pathway which is the desaturation of LA (18:2n-6) and ALA (18:3n-3) into gamma-linoleate (GLA) (18:3n-6) and stearidonate (18:4n-3), respectively (By similarity).
Subsequently, in the biosynthetic pathway of HUFA n-3 series, it desaturates tetracosapentaenoate (24:5n-3) to tetracosahexaenoate (24:6n-3), which is then converted to docosahexaenoate (DHA)(22:6n-3), an important lipid for nervous system function (By similarity).
It can also desaturate (11E)-octadecenoate (trans-vaccenoate, a metabolite in the biohydrogenation pathway of LA and the predominant trans fatty acid in cow milk) at carbon 6 generating (6Z,11E)-octadecadienoate (By similarity).
In addition to Delta-6 activity, this enzyme exhibits Delta-8 activity with slight biases toward n-3 fatty acyl-CoA substrates (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Acyl-CoA desaturase
Regulatory Status
For Research Use Only
