CAMP (Cyclic adenosine monophosphate) ELISA Kit
CAMP (Cyclic adenosine monophosphate) ELISA Kit
The CAMP (Cyclic adenosine monophosphate) ELISA Kit measures cAMP in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 1h assay time. The microplate provided in this kit has been precoated with anti cAMP antibody. Sample or standard completely mixes with fixed amount of HRP labeled cAMP working solution and also add into each well. cAMP in the sample or standard competes with biotin-labeled cAMP to bind with antibody precoated on the microplate. Free components are washed off. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of cAMP in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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