Canine anti- (Chicken Ovomucoid) IgM Elisa kit
Canine anti- (Chicken Ovomucoid) IgM Elisa kit
The Canine anti- (Chicken Ovomucoid) IgM Elisa kit measures OVM-IgM in Canine Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit was based on capture enzyme-linked immune-sorbent assay technology. Anti Canine IgM (u chain) was pre-coated onto the 96-well plate. The HRP conjugated antigen was used as the detection antigen. The standards and pilot samples were added to the wells subsequently. After incubation, unbound conjugates were removed by wash buffer. Then, HRP detection antigen was added to bind with OVM-IgM conjugated on Anti Canine IgM (u chain). After washing, TMB substrates were added to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding acidic stop solution. Read the O.D. absorbance at 450nm in a microplate reader. The concentration of OVM-IgM in the
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