The Canine (CFTR) Cystic Fibrosis Transmembrane Conductance Regulator ELISA Kit measures Cystic fibrosis transmembrane conductance regulator in Canine samples. The plate has been pre-coated with Canine CFTR antibody. CFTR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine CFTR Antibody is added and binds to CFTR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CFTR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine CFTR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundEpithelial ion channel that plays an important role in the regulation of epithelial ion and water transport and fluid homeostasis. Mediates the transport of chloride ions across the cell membrane (By similarity).
Channel activity is coupled to ATP hydrolysis. The ion channel is also permeable to HCO3-; selectivity depends on the extracellular chloride concentration. Exerts its function also by modulating the activity of other ion channels and transporters. Contributes to the regulation of the pH and the ion content of the epithelial fluid layer. Modulates the activity of the epithelial sodium channel (ENaC) complex, in part by regulating the cell surface expression of the ENaC complex. May regulate bicarbonate secretion and salvage in epithelial cells by regulating the transporter SLC4A7. Can inhibit the chloride channel activity of ANO1 (By similarity).
Plays a role in the chloride and bicarbonate homeostasis during sperm epididymal maturation and capacitation (By similarity). Source: UniProt Consortium (2025)
