The Canine Cystatin C ELISA is an enzyme immunoassay for the quantitative determination of Canine Cystatin C in canine serum and urine.
The microtiter plate is coated with the antibody specifically binding the Canine Cystatin C. The Canine serum or urine is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with HRP-labelled detection antibody. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of Canine Cystatin C in the specimen. The concentration of Cystatin C in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.
Catalog No:BA2008
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Product Details
Species ReactivityCanine
Sensitivity5 pg/mL
Detection Range0.313 - 10 ng/mL
Sample TypeSerum, Urine
Sample Size5 uL
Incubation(s)2 hour(s)
Research AreasNephrology
BackgroundCystatin C is a non-glycosylated basic protein belonging to the super-family of cysteine proteinase inhibitors. It consists of a single polypeptide chain having 120 amino acids.
It is produced by all nucleated cells within the body and is released during phagocytosis and inflammation. In the kidney, cystatin C is freely filtrated through the glomerulus and reabsorbed and catabolized in the proximal renal tubules. The rate of cystatin C synthesis is constant, independent of age, gender and muscle mass. High concentrations can be found in serum, seminal fluid, cerebrospinal fluid (CSF), and synovial fluid, and lower concentrations can be found in urine.
In human medicine, cystatin C is the most important endogenous serum marker of renal function assessment. Cystatin C evaluation is able to detect an earlier stage of decreased glomerular filtration rate (GFR) than other parameters (serum creatinine, creatinine clearance etc.) and it is considered particularly useful in patients with a high risk of developing nephropathies. Imbalance between cystatin C and cysteine proteinases is associated with inflammation, cancer, Alzheimer's disease, multiple sclerosis and hereditary cystatin C amyloid angiopathy. An increased level has been found in patients with autoimmune diseases. On the other hand, low concentration of cystatin C presents a risk factor for secondary cardiovascular events.
In veterinary medicine, there are multiple reports of the use of cystatin C in the evaluation of renal function indicating that cystatin C is also the most important serum (urine) marker of renal function assessment in dogs.
