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Canine ET-1 (Endothelin 1) ELISA Kit

Canine ET-1 (Endothelin 1) ELISA Kit

The Canine (ET-1) Endothelin 1 ELISA Kit measures Endothelin 1 in Canine samples. The plate has been pre-coated with Canine ET-1 antibody. ET-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine ET-1 Antibody is added and binds to ET-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ET-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine ET-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0014Ca
Regular price $618.00 USD
Regular price $475.00 USD Sale price $618.00 USD
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2.5 weeks
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Product Details

Species Reactivity Canine
Sensitivity 0.53 ng/L
Detection Range 1-400 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Cardiovascular
Background Endothelins are endothelium-derived vasoconstrictor Peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Endothelin 1
Regulatory Status For Research Use Only
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