The Canine/Feline Clusterin ELISA is an enzyme immunoassay for the quantitative determination of canine/feline clusterin in urine, serum and plasma.
In the Canine/Feline Clusterin ELISA, standards, quality controls and samples are incubated in microplate wells pre-coated with specific anti-canine/feline clusterin antibody. After 60 minutes incubation and washing, biotin labelled anti-canine/feline clusterin antibody is added and incubated with captured clusterin for 60 minutes. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of clusterin. A standard curve is constructed by plotting absorbance values against concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
Catalog No:BA2007
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Product Details
Species ReactivityCanine, Feline
Sensitivity0.2 ng/mL
Detection Range1 - 40 ng/mL
Sample TypeSerum, Plasma, Urine
Sample Size5 to 10 uL
Incubation(s)2.5 hour(s)
Research AreasNephrology
BackgroundClusterin, also known as apolipoprotein J, is a widely expressed heterodimeric glycoprotein, important in tumorigenesis, apoptosis and immunoregulation. Clusterin is a cellular chaperon that stabilizes stressed proteins in a folding-competent state and protein has also been implicated in programmed cell death. Another defining prominent of clusterin is its induction in many severe physiological disturbance states including kidney degenerative diseases, prostate and vesicle carcinogenesis, ovarian cancer, and several neurodegenerative conditions (Alzheimer’s disease).
Recent findings demonstrate that high serum clusterin levels are connected to oxidation stress, vascular damage, sepsis and related mortality or significantly lower serum clusterin was observed in dogs with multicentric lymphoma (MLSA).
Urinary clusterin may also help to differentiate between tubular and glomerular forms of proteinuria.
