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Canine IL-21 (Interleukin 21) ELISA Kit

Canine IL-21 (Interleukin 21) ELISA Kit

The Canine (IL-21) Interleukin 21 ELISA Kit measures Interleukin 21 in Canine samples. The plate has been pre-coated with Canine IL21 antibody. IL21 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Canine IL21 Antibody is added and binds to IL21 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL21 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Canine IL21. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0075Ca
Regular price $618.00 USD
Regular price $475.00 USD Sale price $618.00 USD
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Packaging
2.5 weeks
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Product Details

Species Reactivity Canine
Sensitivity 1.02 ng/L
Detection Range 2-600 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology, Cancer, Cytokines, Inflammation
Background Cytokine with immunoregulatory activity. May promote the transition between innate and adaptive immunity. Induces the production of IgG(1) and IgG(3) in B-cells. Implicated in the generation and maintenance of T follicular helper (Tfh) cells and the formation of germinal-centers. Together with IL6, control the early generation of Tfh cells and are critical for an effective antibody response to acute viral infection (By similarity). May play a role in proliferation and maturation of natural killer (NK) cells in synergy with IL15. May regulate proliferation of mature B- and T-cells in response to activating stimuli. In synergy with IL15 and IL18 stimulates interferon gamma production in T-cells and NK cells (By similarity). During T-cell mediated immune response may inhibit dendritic cells (DC) activation and maturation (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Interleukin 21
Regulatory Status For Research Use Only
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