The Canine Procalcitonin ELISA is an enzyme immunoassay for the quantitative determination of Procalcitonin in canine serum, plasma and urine.
In the Canine Procalcitonin ELISA kit, standards and samples are incubated in microtitration wells pre-coated with polyclonal anti-canine procalcitonin antibody. After 60 minutes incubation followed by washing, biotin-labelled polyclonal anti-canine procalcitonin antibody is added and incubated with the captured procalcitonin for 60 minutes. After another washing, streptavidin-HRP conjugate is added. After 30 minutes incubation and the last washing step, the remaining conjugate is allowed to react with the substrate solution (TMB). The reaction is stopped by addition of acidic solution and absorbance of the resulting yellow product is measured. The absorbance is proportional to the concentration of canine procalcitonin. A standard curve is constructed by plotting absorbance values against procalcitonin concentrations of standards and concentrations of unknown samples are determined using this standard curve.
Catalog No:BA2006
Regular price
$560.00 USD
Regular price
$560.00 USD
Sale price
$560.00 USD
Unit price/ per
Sale
Sold out
2 weeks
Couldn't load pickup availability
Product Details
Species ReactivityCanine
Sensitivity4 pg/mL
Detection Range25 - 800 pg/mL
Sample TypeSerum, Plasma, Urine
Sample Size20 uL
Incubation(s)2.5 hour(s)
Research AreasInfection immunity
BackgroundProcalcitonin (PCT), a polypeptide with a molecular mass of about 13 kDa, is the precursor of calcitonin. PCT is constitutively produced in the C cells of the thyroid gland and does not exhibit hormone activity.
PCT is markedly elevated within 2 to 4 hours in severe forms of systemic inflammation or in bacterial infections, and the level persists until recovery. The biological half-life of PCT is 22 to 26 hours, an advantageous time point compared with CRP and other acute-phase reactants [2]. Because up-regulation of PCT is attenuated by INF- gamma, a cytokine released in response to viral infections, PCT is more specific for bacterial infections and may help to distinguish bacterial infections from viral illnesses.
It should be noted that PCT is also elevated in noninfectious conditions such as trauma, surgery, cardiogenic shock, burns, heat stroke, acute respiratory distress syndrome, infected necrosis after acute pancreatitis, and rejection after transplantation.
Severe bacterial infections can result in marked morbidity and death in veterinary patients, with 50–70% of dogs with sepsis succumbing to their disease. Early diagnosis of infection is essential for the appropriate management of sepsis, as it allows rapid administration of antibiotics resulting in improved outcomes. Although PCT mRNA expression from nonthyroidal tissue has been demonstrated in dogs with inflammation, sepsis and SIRS, very little is known about serum PCT concentration in dogs due to the lack of a validated canine assay.
