CERAMIDE ELISA Kit
CERAMIDE ELISA Kit
The CERAMIDE ELISA Kit measures Ceramide in Universal Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Competitive-ELISA detection method and takes 2h assay time. The microplate provided in this kit has been precoated with Ceramide. Ceramide in the sample or standard competes with a fixed amount of biotin-labeled Ceramide for sites on a pre-coated antibody specific to Ceramide. Free components are washed off. After adding HRP-Streptavidin Conjugate (SABC), biotin specifically binds with streptavidin to form immune complexes. Wash off unbound conjugate. Add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Compare OD450 value of sample with the standard curve through curve fitting software to determine the concentration of Ceramide in the sample. The concentration of the target substance is inversely proportional to the OD450 value.
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