Cys-C (Cystatin C) ELISA Kit
Cys-C (Cystatin C) ELISA Kit
This Cys-C (Cystatin C) ELISA Kit uses the sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human Cys-C. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human Cys-C are added to the micro ELISA plate wells. Human Cys-C in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human Cys-C.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human Cys-C. The concentration of Human Cys-C in the samples is then determined by comparing the OD of the samples to the standard curve.
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