• D-Dimer ELISA
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D-Dimer ELISA

D-Dimer ELISA

The D-Dimer ELISA is an enzyme immunoassay for the quantitative determination of D-dimer in human serum and plasma.

The microtiter plate is coated with the antibody specifically binding the D-dimer. The human serum or plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of D-dimer in the specimen. The concentration of D-dimer in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA1005
$560.00 USD
$560.00 USD $560.00 USD
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Packaging
2 weeks

Product Details

Species Reactivity Human
Sensitivity 1.25 ng/mL
Detection Range 5 - 160 ng/mL
Sample Type Serum, Plasma
Sample Size 50 uL
Incubation(s) 2.5 hour(s)
Research Areas Cardiovascular
Background D-dimer, the final degradation product of cross-linked fibrin, is typically elevated in patients with acute venous thromboembolism.1 D-dimer testing is an integral part of validated algorithms for the diagnosis of deep-vein thrombosis (DVT) and pulmonary embolism (PE).
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte D-Dimer
Regulatory Status For Research Use Only
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