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E2 (Estradiol) ELISA Kit
E2 (Estradiol) ELISA Kit
The E2 (Estradiol) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with E2. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to E2. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No:
RE10177
Regular price
$450.00 USD
Regular price
$450.00 USD
Sale price
$450.00 USD
Unit price
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per
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Product Details
Species Reactivity
Universal
Sensitivity
7.5 pg/mL
Detection Range
12.5-800 pg/mL
Sample Type
Serum, Plasma, other biological fluids
Sample Size
50 uL
Incubation(s)
1.5 hour(s)
Storage Condition and Shelf Life
Unopened store at 2-8C for 12 months.
Analyte
Estradiol
Regulatory Status
For Research Use Only
