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Equine IGF-1 (Insulin-like Growth Factors 1) ELISA Kit
Equine IGF-1 (Insulin-like Growth Factors 1) ELISA Kit
The Equine (IGF-1) Insulin-like Growth Factors 1 ELISA Kit measures Insulin-like growth factors 1 in Equine samples. The plate has been pre-coated with Equine IGF-1 antibody. IGF-1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Equine IGF-1 Antibody is added and binds to IGF-1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IGF-1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Equine IGF-1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0014HO
Regular price
$647.00 USD
Regular price
$498.00 USD
Sale price
$647.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Equine
Sensitivity
3.52 ng/mL
Detection Range
7-1500 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Cancer, Developmental Biology, Diabetes Research, Endocrinology, Metabolism
Background
The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation. Ca2+-dependent exocytosis of IGF1 is required for sensory perception of smell in the olfactory bulb. Acts as a ligand for IGF1R. Binds to the alpha subunit of IGF1R, leading to the activation of the intrinsic tyrosine kinase activity which autophosphorylates tyrosine residues in the beta subunit thus initiatiating a cascade of down-stream signaling events leading to activation of the PI3K-AKT/PKB and the Ras-MAPK pathways. Binds to integrins ITGAV:ITGB3 and ITGA6:ITGB4. Its binding to integrins and subsequent ternary complex formation with integrins and IGFR1 are essential for IGF1 signaling. Induces the phosphorylation and activation of IGFR1, MAPK3/ERK1, MAPK1/ERK2 and AKT1 (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Insulin-like growth factors 1
Regulatory Status
For Research Use Only
