The Equine (SERPINB1) Leukocyte Elastase Inhibitor ELISA Kit measures Leukocyte Elastase Inhibitor in Equine samples. The plate has been pre-coated with Equine SERPINB1 antibody. SERPINB1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Equine SERPINB1 Antibody is added and binds to SERPINB1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SERPINB1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Equine SERPINB1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundNeutrophil serine protease inhibitor that plays an essential role in the regulation of the innate immune response, inflammation and cellular homeostasis. Acts primarily to protect the cell from proteases released in the cytoplasm during stress or infection. These proteases are important in killing microbes but when released from granules, these potent enzymes also destroy host proteins and contribute to mortality. Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. Acts also as a potent intracellular inhibitor of GZMH by directly blocking its proteolytic activity. During inflammation, limits the activity of inflammatory caspases CASP1, CASP4 and CASP5 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation. When secreted, promotes the proliferation of beta-cells via its protease inhibitory function. Source: UniProt Consortium (2025)
