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Feline IL-10 (Interleukin 10) ELISA Kit
Feline IL-10 (Interleukin 10) ELISA Kit
The Feline (IL-10) Interleukin 10 ELISA Kit measures Interleukin 10 in Feline samples. The plate has been pre-coated with Feline IL-10 antibody. IL-10 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Feline IL-10 Antibody is added and binds to IL-10 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-10 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Feline IL-10. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0030Cat
Regular price
$618.00 USD
Regular price
$475.00 USD
Sale price
$618.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Feline
Sensitivity
10.51 ng/L
Detection Range
20-4000 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology, Cytokines, Inflammation
Background
Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators. Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha. Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (By similarity).
In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Interleukin 10
Regulatory Status
For Research Use Only
