• FGF-23 ELISA
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FGF-23 ELISA

FGF-23 ELISA

The Intact Fibroblast Growth Factor 23 (FGF-23) ELISA is an enzyme immunoassay for the quantitative determination of Intact Fibroblast Growth Factor 23 (FGF-23) in human EDTA plasma.

The microtiter plate is coated with the antibody specifically binding Fibroblast growth factor 23. The human EDTA plasma is incubated in the plate with the capture antibody. The specimen is washed out and the specifically bound protein is incubated with biotin-labelled detection antibody. Following another washing step, Streptavidin-HRP conjugate is added into the well. Unbound reagent is then washed out. Horseradish peroxidase (HRP) bound in the complex reacts with the chromogenic substrate (TMB) creating the blue colour. The reaction is stopped by addition of STOP solution (H2SO4). The absorbance values are measured at 450 nm (optionally 450/630 nm) and are proportional to the concentration of FGF23 in the specimen. The concentration of FGF23 in unknown samples is determined from the calibration curve which is created by plotting the absorbance values against the standard concentration values.

Catalog No: BA1019
$560.00 USD
$560.00 USD $560.00 USD
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2 weeks

Product Details

Species Reactivity Human
Sensitivity 25 pg/mL
Detection Range 37.5 - 2400 pg/mL
Sample Type Plasma
Sample Size 75 uL
Incubation(s) ON + 2.5 hour(s)
Research Areas Endocrinology
Background Fibroblast growth factor-23 (FGF-23) is a phosphaturic hormone involved in mineral bone metabolism that helps control phosphate homeostasis and reduces 1,25- dihydroxyvitamin D synthesis. Recent data have highlighted the relevant direct FGF-23 effects on the myocardium, and high plasma levels of FGF-23 have been associated with adverse cardiovascular outcomes in humans, such as heart failure and arrhythmias. Therefore, FGF-23 has emerged as a novel biomarker of cardiovascular risk. Experimental data suggest FGF-23 as a direct mediator of cardiac hypertrophy development, cardiac fibrosis and cardiac dysfunction via specific myocardial FGF receptor (FGFR) activation.
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Fibroblast Growth Factors
Regulatory Status For Research Use Only
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