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Fish TSH (Thyroid Stimulating Hormone) ELISA Kit

Fish TSH (Thyroid Stimulating Hormone) ELISA Kit

The Fish (TSH) Thyroid Stimulating Hormone ELISA Kit measures Thyroid Stimulating Hormone in Fish samples. Add samples to the pre-coated plate. Then add biotinylated antigen. The antigens in the samples compete with the biotinylated antigen to bind to the capture antibody and incubate. Unbound antigen is washed away during a washing step. An avidin-HRP is then added and then incubate. Unbound avidin-HRP is washed away during a washing step. TMB Substrate is then added and color develops. The reaction is stopped by addition of acidic stop solution and color changes into yellow that can be measured at 450 nm. The intensity of the color developed is inversely proportional to the concentration of TSH in the sample. The concentration of TSH in the sample is then determined by comparing the O.D. of the samples to the standard curve.

Catalog No: EA0028Fi
Regular price $647.00 USD
Regular price $498.00 USD Sale price $647.00 USD
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Packaging
2.5 weeks
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Product Details

Species Reactivity Fish
Sensitivity 0.13 uIU/mL
Detection Range 0.3 - 90 uIU/mL
Sample Type Serum, plasma, cell culture supernatant
Incubation(s) 2.5 hour(s)
Research Areas Signal Transduction, Endocrinology
Background Thyroid-stimulating hormone (also known as thyrotropin, thyrotropic hormone, or abbreviated TSH) is a pituitary hormone that stimulates the thyroid gland to produce thyroxine (T4), and then triiodothyronine (T3) which stimulates the metabolism of almost every tissue in the body.[1] It is a glycoprotein hormone produced by thyrotrope cells in the anterior pituitary gland, which regulates the endocrine function of the thyroid. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Thyroid Stimulating Hormone
Regulatory Status For Research Use Only
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