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Goat IL-1B (Interleukin 1 Beta) ELISA Kit

Goat IL-1B (Interleukin 1 Beta) ELISA Kit

The Goat (IL-1B) Interleukin 1 Beta ELISA Kit measures Interleukin 1Beta in Goat samples. The plate has been pre-coated with Goat IL-1beta antibody. IL-1beta present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Goat IL-1beta Antibody is added and binds to IL-1beta in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated IL-1beta antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Goat IL-1beta. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E0027GO
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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Packaging
2.5 weeks
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Product Details

Species Reactivity Goat
Sensitivity 0.51 ng/L
Detection Range 1-300 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Immunology, Cytokines, Inflammation
Background Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Interleukin 1Beta
Regulatory Status For Research Use Only
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