Guinea pig NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit
Guinea pig NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA Kit
The Guinea pig NADPH (Nicotinamide Adenine Dinucleotide Phosphate) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Guinea pig NADPH. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to Guinea pig NADPH. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig NADPH in the samples is then determined by comparing the OD of the samples to the standard curve.
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