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HA (Histamine) ELISA Kit
HA (Histamine) ELISA Kit
The HA (Histamine) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with HA. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to HA. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HA in the samples is then determined by comparing the OD of the samples to the standard curve.
Catalog No:
RE10142
Regular price
$450.00 USD
Regular price
$450.00 USD
Sale price
$450.00 USD
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Product Details
Species Reactivity
Universal
Sensitivity
0.51 ng/mL
Detection Range
1.57-100 ng/mL
Sample Type
Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Incubation(s)
1.5 hour(s)
Storage Condition and Shelf Life
Unopened store at 2-8C for 12 months.
Analyte
Histamine
Regulatory Status
For Research Use Only
