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Human ABCA2 (Atp-binding Cassette Sub-family A Member 2) ELISA Kit

Human ABCA2 (Atp-binding Cassette Sub-family A Member 2) ELISA Kit

The Human (ABCA2) Atp-binding Cassette Sub-family A Member 2 ELISA Kit measures Atp-Binding Cassette Sub-Family A Member 2 in samples. The plate has been pre-coated with Human ABCA2 antibody. ABCA2 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ABCA2 Antibody is added and binds to ABCA2 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ABCA2 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ABCA2. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E2421Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 4.57 ng/L
Detection Range 10-2000 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Probable lipid tranporter that modulates cholesterol sequestration in the late endosome/lysosome by regulating the intracellular sphingolipid metabolism, in turn participates in cholesterol homeostasis (PubMed: 15238223, PubMed: 21810484, PubMed: 24201375) (Probable). May alter the transbilayer distribution of ceramide in the intraluminal membrane lipid bilayer, favoring its retention in the outer leaflet that results in increased acid ceramidase activity in the late endosome/lysosome, facilitating ceramide deacylation to sphingosine leading to the sequestration of free cholesterol in lysosomes (PubMed: 24201375). In addition regulates amyloid-beta production either by activating a signaling pathway that regulates amyloid precursor protein transcription through the modulation of sphingolipid metabolism or through its role in gamma-secretase processing of APP (PubMed: 22086926, PubMed: 26510981). May play a role in myelin formation (By similarity). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Atp-Binding Cassette Sub-Family A Member 2
Regulatory Status For Research Use Only
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