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Human AMACR (Alpha-methylacyl-CoA Recemase) ELISA Kit

Human AMACR (Alpha-methylacyl-CoA Recemase) ELISA Kit

The Human (AMACR) Alpha-methylacyl-CoA Recemase ELISA Kit measures Alpha-Methylacyl-Coa Recemase in samples. The plate has been pre-coated with Human AMACR antibody. AMACR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human AMACR Antibody is added and binds to AMACR in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated AMACR antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human AMACR. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E3387Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 10.16 ng/L
Detection Range 20-4500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Background Catalyzes the interconversion of (R)- and (S)-stereoisomers of alpha-methyl-branched-chain fatty acyl-CoA esters (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359). Acts only on coenzyme A thioesters, not on free fatty acids, and accepts as substrates a wide range of alpha-methylacyl-CoAs, including pristanoyl-CoA, trihydroxycoprostanoyl-CoA (an intermediate in bile acid synthesis), and arylpropionic acids like the anti-inflammatory drug ibuprofen (2-(4-isobutylphenyl)propionic acid) but neither 3-methyl-branched nor linear-chain acyl-CoAs (PubMed: 7649182, PubMed: 10655068, PubMed: 11060359). Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Alpha-Methylacyl-Coa Recemase
Regulatory Status For Research Use Only
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