Human Anti- B. burgdorferi (Borreliella burgdorferi) IgM ELISA Kit
Human Anti- B. burgdorferi (Borreliella burgdorferi) IgM ELISA Kit
The Human Anti- B. burgdorferi (Borreliella burgdorferi) IgM ELISA Kit measures anti-B. burgdorferi IgM in Human Serum, Plasma. This kit is based on capture ELISA detection method and takes 3h assay time. The microplate provided in this kit has been precoated with anti Human IgM(U-chain). HRP-antigen is used as detection antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add HRP-antigen. Then, it binds with anti-B. burgdorferi IgM bound to anti Human IgM(U-chain). Wash unbound components and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of anti-B. burgdorferi IgM in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.
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