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Human APO-E4 (Apolipoprotein E4) ELISA Kit
Human APO-E4 (Apolipoprotein E4) ELISA Kit
The Human (APO-E4) Apolipoprotein E4 ELISA Kit measures Apolipoprotein E4 in samples. The plate has been pre-coated with Human APOE antibody. APOE present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human APOE Antibody is added and binds to APOE in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated APOE antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human APOE. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E4761Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.12 ug/mL
Detection Range
0.2-60 ug/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Signal Transduction, Cardiovascular
Background
APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids (PubMed: 6860692, PubMed: 1911868, PubMed: 1$475.00|$350.004908). APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance (PubMed: 6860692, PubMed: 2762297, PubMed: 1911868, PubMed: 1917954, PubMed: 9395455, PubMed: 1$475.00|$350.004908, PubMed: 23620513). Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma (PubMed: 6860692, PubMed: 2762297, PubMed: 9395455). As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL) (PubMed: 6860692, PubMed: 1911868). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles (PubMed: 2762297, PubMed: 1917954, PubMed: 7768901, PubMed: 8939961, PubMed: 12950167, PubMed: 20030366, PubMed: 2063194, PubMed: 8756331, PubMed: 20303980, PubMed: 1530612, PubMed: 7635945). Finally, APOE has also a heparin-binding activity and binds heparan-sulfate proteoglycans on the surface of cells, a property that supports the capture and the receptor-mediated uptake of APOE-containing lipoproteins by cells (PubMed: 9395455, PubMed: 9488694, PubMed: 23676495, PubMed: 7635945). A main function of APOE is to mediate lipoprotein clearance through the uptake of chylomicrons, VLDLs, and HDLs by hepatocytes (PubMed: 1911868, PubMed: 1917954, PubMed: 9395455, PubMed: 23676495, PubMed: 29516132). APOE is also involved in the biosynthesis by the liver of VLDLs as well as their uptake by peripheral tissues ensuring the delivery of triglycerides and energy storage in muscle, heart and adipose tissues (PubMed: 2762297, PubMed: 29516132). By participating in the lipoprotein-mediated distribution of lipids among tissues, APOE plays a critical role in plasma and tissues lipid homeostasis (PubMed: 2762297, PubMed: 1917954, PubMed: 29516132). APOE is also involved in two steps of reverse cholesterol transport, the HDLs-mediated transport of cholesterol from peripheral tissues to the liver, and thereby plays an important role in cholesterol homeostasis (PubMed: 9395455, PubMed: 1$475.00|$350.004908, PubMed: 23620513). First, it is functionally associated with ABCA1 in the biogenesis of HDLs in tissues (PubMed: 1$475.00|$350.004908, PubMed: 23620513). Second, it is enriched in circulating HDLs and mediates their uptake by hepatocytes (PubMed: 9395455). APOE also plays an important role in lipid transport in the central nervous system, regulating neuron survival and sprouting (PubMed: 8939961, PubMed: 25173806). APOE in also involved in innate and adaptive immune responses, controlling for instance the survival of myeloid-derived suppressor cells (By similarity). APOE, may also play a role in transcription regulation through a receptor-dependent and cholesterol-independent mechanism, that activates MAP3K12 and a non-canonical MAPK signal transduction pathway that results in enhanced AP-1-mediated transcription of APP (PubMed: 28111074). (Microbial infection) Through its interaction with HCV envelope glycoprotein E2, participates in the attachment of HCV to HSPGs and other receptors (LDLr, VLDLr, and SR-B1) on the cell surface and to the assembly, maturation and infectivity of HCV viral particles (PubMed: 25122793, PubMed: 29695434). This interaction is probably promoted via the up-regulation of cellular autophagy by the virus (PubMed: 29695434). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Apolipoprotein E4
Regulatory Status
For Research Use Only
