Human ARHGEF5 (Rho Guanine Nucleotide Exchange Factor 5) ELISA Kit
Human ARHGEF5 (Rho Guanine Nucleotide Exchange Factor 5) ELISA Kit
The Human (ARHGEF5) Rho Guanine Nucleotide Exchange Factor 5 ELISA Kit measures Rho Guanine Nucleotide Exchange Factor 5 in samples. The plate has been pre-coated with Human ARHGEF5 antibody. ARHGEF5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ARHGEF5 Antibody is added and binds to ARHGEF5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ARHGEF5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ARHGEF5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
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