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Human ASAH1 (Acid Ceramidase) ELISA Kit
Human ASAH1 (Acid Ceramidase) ELISA Kit
The Human (ASAH1) Acid Ceramidase ELISA Kit measures Acid Ceramidase in samples. The plate has been pre-coated with Human ASAH1 antibody. ASAH1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ASAH1 Antibody is added and binds to ASAH1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ASAH1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ASAH1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E3476Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.28 ng/mL
Detection Range
0.5-180 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Background
Lysosomal ceramidase that hydrolyzes sphingolipid ceramides into sphingosine and free fatty acids at acidic pH (PubMed: 10610716, PubMed: 7744740, PubMed: 15655246, PubMed: 11451951). Ceramides, sphingosine, and its phosphorylated form sphingosine-1-phosphate are bioactive lipids that mediate cellular signaling pathways regulating several biological processes including cell proliferation, apoptosis and differentiation (PubMed: 10610716). Has a higher catalytic efficiency towards C12-ceramides versus other ceramides (PubMed: 7744740, PubMed: 15655246). Also catalyzes the reverse reaction allowing the synthesis of ceramides from fatty acids and sphingosine (PubMed: 12764132, PubMed: 12815059). For the reverse synthetic reaction, the natural sphingosine D-erythro isomer is more efficiently utilized as a substrate compared to D-erythro-dihydrosphingosine and D-erythro-phytosphingosine, while the fatty acids with chain lengths of 12 or 14 carbons are the most efficiently used (PubMed: 12764132). Has also an N-acylethanolamine hydrolase activity (PubMed: 15655246). By regulating the levels of ceramides, sphingosine and sphingosine-1-phosphate in the epidermis, mediates the calcium-induced differentiation of epidermal keratinocytes (PubMed: 17713573). Also indirectly regulates tumor necrosis factor/TNF-induced apoptosis (By similarity). By regulating the intracellular balance between ceramides and sphingosine, in adrenocortical cells, probably also acts as a regulator of steroidogenesis (PubMed: 22261821). [Isoform 2]: May directly regulate steroidogenesis by binding the nuclear receptor NR5A1 and negatively regulating its transcriptional activity. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Acid Ceramidase
Regulatory Status
For Research Use Only
