The Human (ASPM) Abnormal Spindle-like Microcephaly-associated Protein ELISA Kit measures Abnormal Spindle-Like Microcephaly-Associated Protein in samples. The plate has been pre-coated with Human ASPM antibody. ASPM present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ASPM Antibody is added and binds to ASPM in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ASPM antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ASPM. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundInvolved in mitotic spindle regulation and coordination of mitotic processes. The function in regulating microtubule dynamics at spindle poles including spindle orientation, astral microtubule density and poleward microtubule flux seems to depend on the association with the katanin complex formed by KATNA1 and KATNB1. Enhances the microtubule lattice severing activity of KATNA1 by recruiting the katanin complex to microtubules. Can block microtubule minus-end growth and reversely this function can be enhanced by the katanin complex (PubMed: 28436967). May have a preferential role in regulating neurogenesis. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteAbnormal Spindle-Like Microcephaly-Associated Protein
