The Human (ATP2B1) Plasma Membrane Calcium-transporting Atpase 1 ELISA Kit measures Plasma membrane calcium-transporting ATPase 1 in samples. The plate has been pre-coated with Human ATP2B1 antibody. ATP2B1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATP2B1 Antibody is added and binds to ATP2B1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATP2B1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ATP2B1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundCatalyzes the hydrolysis of ATP coupled with the transport of calcium from the cytoplasm to the extracellular space thereby maintaining intracellular calcium homeostasis. Plays a role in blood pressure regulation through regulation of intracellular calcium concentration and nitric oxide production leading to regulation of vascular smooth muscle cells vasoconstriction. Positively regulates bone mineralization through absorption of calcium from the intestine. Plays dual roles in osteoclast differentiation and survival by regulating RANKL-induced calcium oscillations in preosteoclasts and mediating calcium extrusion in mature osteoclasts (By similarity).
Regulates insulin sensitivity through calcium/calmodulin signaling pathway by regulating AKT1 activation and NOS3 activation in endothelial cells (PubMed:29104511).
May play a role in synaptic transmission by modulating calcium and proton dynamics at the synaptic vesicles. Source: UniProt Consortium (2025)
