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Human ATP5A1 (Atp Synthase Subunit Alpha) ELISA Kit
Human ATP5A1 (Atp Synthase Subunit Alpha) ELISA Kit
The Human (ATP5A1) Atp Synthase Subunit Alpha ELISA Kit measures ATP Synthase subunit alpha in samples. The plate has been pre-coated with Human ATP5A1 antibody. ATP5A1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATP5A1 Antibody is added and binds to ATP5A1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATP5A1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ATP5A1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6762Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
3.67 ng/L
Detection Range
7-1500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Tags and Cell Markers, Signal Transduction, Metabolism
Background
ATP synthase F1 subunit alpha, mitochondrial is an enzyme that in humans is encoded by the ATP5F1A gene. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the same protein have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
ATP Synthase subunit alpha
Regulatory Status
For Research Use Only
