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Human ATP5J (Atp Synthase-Coupling Factor 6) ELISA Kit
Human ATP5J (Atp Synthase-Coupling Factor 6) ELISA Kit
The Human (ATP5J) Atp Synthase-Coupling Factor 6 ELISA Kit measures ATP Synthase-coupling factor 6 in samples. The plate has been pre-coated with Human ATP5J antibody. ATP5J present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human ATP5J Antibody is added and binds to ATP5J in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated ATP5J antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human ATP5J. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E6763Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
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per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
3.88 ng/L
Detection Range
7-1500 ng/L
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Tags and Cell Markers, Signal Transduction, Metabolism
Background
ATP synthase-coupling factor 6, mitochondrial is an enzyme subunit that in humans is encoded by the ATP5PF gene. The F6 subunit is part of the peripheral stalk that links the F1 and FO complexes together, and which acts as a stator to prevent certain subunits from rotating with the central rotary element. The peripheral stalk differs in subunit composition between mitochondrial, chloroplast and bacterial F-ATPases. In mitochondria, the peripheral stalk is composed of one copy each of subunits OSCP (oligomycin sensitivity conferral protein), F6, b and d.[8] There is no homologue of subunit F6 in bacterial or chloroplast F-ATPase, whose peripheral stalks are composed of one copy of the delta subunit (homologous to OSCP), and two copies of subunit b in bacteria, or one copy each of subunits b and b' in chloroplasts and photosynthetic bacteria. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
ATP Synthase-coupling factor 6
Regulatory Status
For Research Use Only
