The Human (BCAR3) Breast Cancer Anti-Estrogen Resistance Protein 3 ELISA Kit measures Breast Cancer Anti-Estrogen Resistance Protein 3 in samples. The plate has been pre-coated with Human BCAR3 antibody. BCAR3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human BCAR3 Antibody is added and binds to BCAR3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BCAR3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human BCAR3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Research AreasEndocrinology, Hormone Metabolism, Cancer, Oncology
BackgroundActs as an adapter protein downstream of several growth factor receptors to promote cell proliferation, migration, and redistribution of actin fibers (PubMed: 24216110). Specifically involved in INS/insulin signaling pathway by mediating MAPK1/ERK2-MAPK3/ERK1 activation and DNA synthesis (PubMed: 24216110). Promotes insulin-mediated membrane ruffling (By similarity). In response to vasoconstrictor Peptide EDN1, involved in the activation of RAP1 downstream of PTK2B via interaction with phosphorylated BCAR1 (PubMed: 19086031). Inhibits cell migration and invasion via regulation of TGFB-mediated matrix digestion, actin filament rearrangement, and inhibition of invadopodia activity (By similarity). May inhibit TGFB-SMAD signaling, via facilitating BCAR1 and SMAD2 and/or SMAD3 interaction (By similarity). Regulates EGF-induced DNA synthesis (PubMed: 18722344). Required for the maintenance of ocular lens morphology and structural integrity, potentially via regulation of focal adhesion complex signaling (By similarity). Acts upstream of PTPRA to regulate the localization of BCAR1 and PTPRA to focal adhesions, via regulation of SRC-mediated phosphorylation of PTPRA (By similarity). Positively regulates integrin-induced tyrosine phosphorylation of BCAR1 (By similarity). Acts as a guanine nucleotide exchange factor (GEF) for small GTPases RALA, RAP1A and RRAS (By similarity). However, in a contrasting study, lacks GEF activity towards RAP1 (PubMed: 22081014). Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteBreast Cancer Anti-Estrogen Resistance Protein 3
