The Human (BHLHE41) Class E Basic Helix-Loop-Helix Protein 41 ELISA Kit measures Class E Basic Helix-Loop-Helix Protein 41 in samples. The plate has been pre-coated with Human BHLHE41 antibody. BHLHE41 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human BHLHE41 Antibody is added and binds to BHLHE41 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BHLHE41 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human BHLHE41. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundTranscriptional repressor involved in the regulation of the circadian rhythm by negatively regulating the activity of the clock genes and clock-controlled genes (PubMed: 11278948, PubMed: 14672706, PubMed: 15193144, PubMed: 15560782, PubMed: 18411297, PubMed: 19786558, PubMed: 25083013). Acts as the negative limb of a novel autoregulatory feedback loop (DEC loop) which differs from the one formed by the PER and CRY transcriptional repressors (PER/CRY loop). Both these loops are interlocked as it represses the expression of PER1 and in turn is repressed by PER1/2 and CRY1/2. Represses the activity of the circadian transcriptional activator: CLOCK-ARNTL/BMAL1 heterodimer by competing for the binding to E-box elements (5'-CACGTG-3') found within the promoters of its target genes (PubMed: 25083013). Negatively regulates its own expression and the expression of DBP and BHLHE41/DEC2. Acts as a corepressor of RXR and the RXR-LXR heterodimers and represses the ligand-induced RXRA/B/G, NR1H3/LXRA, NR1H4 and VDR transactivation activity. Inhibits HNF1A-mediated transactivation of CYP1A2, CYP2E1 AND CYP3A11 (By similarity). Source: UniProt Consortium (2025)
