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Human BPI (Bactericidal Permeability-increasing Protein) ELISA Kit
Human BPI (Bactericidal Permeability-increasing Protein) ELISA Kit
The Human (BPI) Bactericidal Permeability-increasing Protein ELISA Kit measures Bactericidal Permeability-Increasing Protein in samples. The plate has been pre-coated with Human BPI antibody. BPI present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human BPI Antibody is added and binds to BPI in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BPI antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human BPI. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0323Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.28 ng/mL
Detection Range
0.5-150 ng/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Immunology
Background
The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-negative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Bactericidal Permeability-Increasing Protein
Regulatory Status
For Research Use Only
