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Human BRAP (Brca1-associated Protein) ELISA Kit

Human BRAP (Brca1-associated Protein) ELISA Kit

The Human (BRAP) Brca1-associated Protein ELISA Kit measures Brca1-Associated Protein in samples. The plate has been pre-coated with Human BRAP antibody. BRAP present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human BRAP Antibody is added and binds to BRAP in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated BRAP antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human BRAP. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E6565Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
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2.5 weeks
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Product Details

Species Reactivity Human
Sensitivity 10.09 ng/L
Detection Range 20-4500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Epigenetics and Nuclear Signaling
Background Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Brca1-Associated Protein
Regulatory Status For Research Use Only
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