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Human C-C Motif Chemokine 15 (CCL15) ELISA Kit
Human C-C Motif Chemokine 15 (CCL15) ELISA Kit
This ELISA kit is designed to detect Human C-C Motif Chemokine 15 (Human CCL15). The assay plate has been pre-coated with mouse anti-Human MIP-5 monoclonal antibody. When the sample containing MIP-5 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human MIP-5 Antibody is added to the wells and binds to MIP-5 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human MIP-5 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE152
Regular price
$624.00 USD
Regular price
$480.00 USD
Sale price
$624.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.46 pg/mL
Detection Range
1.17-75 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cardiovascular, Immunology, Neuroscience
Background
Chemokines are a family of small chemotactic cytokines, or proteins secreted by cells. Chemokines share the same structure similarities such as small size, and the presence of four cysteine residues in conserved locations in order to form their 3-dimensional shape. Some of the chemokines are considered pro-inflammatory which can be induced to recruit cells of the immune system to a site of infection during an immune response, while others are considered homeostatic and are implied in controlling the migration of cells during normal processes of tissue maintenance and development. There are four members of the chemokine family: C-C kemokines, C kemokines, CXC kemokines and CX3C kemokines. The C-C kemokines have two cysteines nearby the amino terminus. There have been at least 27 distinct members of this subgroup reported for mammals, called C-C chemokine ligands-1 to 28. Chemokine ligand 15 (CCL15), also known as leukotactin-1, MIP5, MIP1 and HCC-2, is a small cytokine belonging to the C-C chemokine family. CCL15 is prevantly expressed in liver, small intestine, colon, and in certain leukocytes and macrophages of the lung. It is chemotactic for neutrophils, monocytes, and lymphocytes and elicits its effects by binding to cell surface chemokine receptors like CCR1 and CCR3.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
C-C motif chemokine 15
Regulatory Status
For Research Use Only
