The Human (C163A) Scavenger Receptor Cysteine-rich Type 1 Protein M130 ELISA Kit measures Scavenger receptor cysteine-rich type 1 protein M130 in samples. The plate has been pre-coated with Human C163A antibody. C163A present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human C163A Antibody is added and binds to C163A in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated C163A antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human C163A. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
BackgroundAcute phase-regulated receptor involved in clearance and endocytosis of hemoglobin/haptoglobin complexes by macrophages and may thereby protect tissues from free hemoglobin-mediated oxidative damage. May play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. Binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner. Exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP*1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP*1S phenotype. Induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1. Isoform 3 exhibits the higher capacity for ligand endocytosis and the more pronounced surface expression when expressed in cells.
After shedding, the soluble form (sCD163) may play an anti-inflammatory role, and may be a valuable diagnostic parameter for monitoring macrophage activation in inflammatory conditions. Source: UniProt Consortium (2025)
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
AnalyteScavenger receptor cysteine-rich type 1 protein M130
