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Human C3 (Complement 3) ELISA Kit
Human C3 (Complement 3) ELISA Kit
The Human (C3) Complement 3 ELISA Kit measures Complement 3 in samples. The plate has been pre-coated with Human C3 antibody. C3 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human C3 Antibody is added and binds to C3 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated C3 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human C3. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
Catalog No:
E0373Hu
Regular price
$595.00 USD
Regular price
$458.00 USD
Sale price
$595.00 USD
Unit price
/
per
2.5 weeks
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Product Details
Species Reactivity
Human
Sensitivity
0.025 mg/mL
Detection Range
0.05-30 mg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
1.5 hour(s)
Research Areas
Cancer
Background
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. [C3-beta-c]: Acts as a chemoattractant for neutrophils in chronic inflammation. [Acylation stimulating protein]: adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed: 8376604, PubMed: 2909530, PubMed: 9059512, PubMed: 10432298, PubMed: 15833747, PubMed: 16333141, PubMed: 19615750). Source: UniProt Consortium (2025)
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
2-8C
Analyte
Complement 3
Regulatory Status
For Research Use Only
