Skip to product information
1 of 1

Human CAPN5 (Calpain-5) ELISA Kit

Human CAPN5 (Calpain-5) ELISA Kit

The Human (CAPN5) Calpain-5 ELISA Kit measures Calpain-5 in samples. The plate has been pre-coated with Human CAPN5 antibody. CAPN5 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CAPN5 Antibody is added and binds to CAPN5 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CAPN5 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CAPN5. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

Catalog No: E6587Hu
Regular price $595.00 USD
Regular price $458.00 USD Sale price $595.00 USD
Sale Sold out
Packaging
2.5 weeks
Download PDF

Product Details

Species Reactivity Human
Sensitivity 9.11 ng/L
Detection Range 20-4500 ng/L
Sample Type Serum, plasma, cell culture supernates
Incubation(s) 1.5 hour(s)
Research Areas Signal Transduction, Cell Biology
Background Calpain-5 is a protein that in humans is encoded by the CAPN5 gene. Calpains are calcium-dependent cysteine proteases involved in signal transduction in a variety of cellular processes. A functional calpain protein consists of an invariant small subunit and 1 of a family of large subunits. CAPN5 is one of the large subunits. Unlike some of the calpains, CAPN5 and CAPN6 lack a calmodulin-like domain IV. Because of the significant similarity to Caenorhabditis elegans sex determination gene tra-3, CAPN5 is also called as HTRA3. Source: UniProt Consortium (2025)
Shipping Condition Shipped on cold gel packs.
Storage Condition and Shelf Life 2-8C
Analyte Calpain-5
Regulatory Status For Research Use Only
View full details

Inquire about this product