This ELISA kit is designed to detect Human Carbonic Anhydrase 12 (Human CA12). The assay plate has been pre-coated with mouse anti-Human carbonic anhydrase XII monoclonal antibody. When the sample containing carbonic anhydrase XII is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human carbonic anhydrase XII Antibody is added to the wells and binds to carbonic anhydrase XII in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human carbonic anhydrase XII present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
BackgroundCarbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
