This ELISA kit is designed to detect Human Carbonic Anhydrase 14 (Human CA14). The assay plate has been pre-coated with mouse anti-Human Carbonic Anhydrase XIV monoclonal antibody. When the sample containing Carbonic Anhydrase XIV is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Carbonic Anhydrase XIV Antibody is added to the wells and binds to Carbonic Anhydrase XIV in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Carbonic Anhydrase XIV present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
BackgroundThe carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. CAXIV is a member of CA family that showed an overall similarity of 29–46% to other active CA isozymes. The highest percentage similarity was with a transmembrane CA isoform, CAXII. The CAXIV was found high concentrations in human heart, brain, liver, and skeletal muscle but lower in the colon, small intestine, urinary bladder, and kidney. No CAXIV mRNA was seen in the salivary gland and pancreas. CAXIV is a likely candidate for the extracellular CA postulated to have an important role in modulating excitatory synaptic transmission in brain.
Shipping ConditionShipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
