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Human Carboxypeptidase A2 (CPA2) ELISA Kit
Human Carboxypeptidase A2 (CPA2) ELISA Kit
This ELISA kit is designed to detect Human Carboxypeptidase A2 (Human CPA2). The assay plate has been pre-coated with mouse anti-Human Carboxypeptidase A2 monoclonal antibody. When the sample containing Carboxypeptidase A2 is added to the plate, it binds to the antibodies coated on the wells. Then, a horseradish peroxidase conjugated mouse anti-Human Carboxypeptidase A2 Antibody is added to the wells and binds to Carboxypeptidase A2 in the sample. After washing the wells, substrate solutions are added, and the color intensity is directly proportional to the amount of Human Carboxypeptidase A2 present. The reaction is stopped by adding an acidic stop solution, and the absorbance is measured at 450 nm.
Catalog No:
BPE095
Regular price
$754.00 USD
Regular price
$580.00 USD
Sale price
$754.00 USD
Unit price
/
per
2 weeks
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Product Details
Species Reactivity
Human
Sensitivity
2.27 pg/mL
Detection Range
12.5-800 pg/mL
Sample Type
Serum, plasma, cell culture supernates
Incubation(s)
3.5 hour(s)
Research Areas
Cell Biology
Background
Carboxypeptidase A2 ( CPA2 ) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
Shipping Condition
Shipped on cold gel packs.
Storage Condition and Shelf Life
This product can be stored at 2-8C.
Analyte
Carboxypeptidase A2
Regulatory Status
For Research Use Only
